Protocol detail

Cell-Type Gene Expression in Prostate

Find Similar Protocols

Cell type-specific gene expression profiles in the prostate gland were assessed with the NCBI GEO dataset GDS1973 [19 (link)], which was generated from four different cell types using an antibody pulldown approach against distinct cell surface-specific markers. There were five biological replicates of each pulldown assay, and the expression profiles were determined using the Affymetrix U133 Plus 2.0 arrays. The expression levels of candidate genes were normalized using the β-Actin (ACTB) gene as the internal control.

Free full text: Click here

Yang M., Li J.C., Tao C., Wu S., Liu B., Shu Q., Li B, & Zhu R. (2021). PAQR6 Upregulation Is Associated with AR Signaling and Unfavorite Prognosis in Prostate Cancers. Biomolecules, 11(9), 1383.

Publication 2021

U133 Plus 2.0 arrays

Actin Assay Biological Cell Cell gene expression profiles Expression genes Gene control Prostate gland Surface antibody

Top 5 similar protocols

Variable analysis

independent variables

Cell type

dependent variables

Gene expression profiles

control variables

β-Actin (ACTB) gene as the internal control

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!