All crosses and staging were performed at 25°C. Stocks are described in FlyBase (http://flybase.bio.indiana.edu ). UAS-YFP-Rab11, UAS-YFP-Rab11Q70L, UAS-YFP-Rab11S25N, UAS-Rab11-RNAi, UAS-Cdc42V12, UAS-YFP-Rab8Q67L, UAS-YFP-Rab4, UAS-YFP-Rab14Q97L, UAS-aPKC-RNAi, UAS-Rho1, Dia5 FRT40A, UAS-YFP-Rab5, GMR-gal4, NufEY11342, Rho172F, Cdc424 FRT19A, UAS-Sec8, Rab11EP3017, UAS-Rok-CAT, UAS-LIMK-HA, UAS-MBS-RNAi, and SqhAX3; RLC-GFP were provided by the Bloomington Stock Center (Indiana University, Bloomington, IN); UAS-Rab5-RNAi was provided by the Vienna Drosophila RNAi Center (Vienna, Austria); UAS-Rok-RNAi and UAS-PKN-RNAi were provided by the National Institute of Genetics (Mishima, Japan); ShgR69 FRT42D was provided by C. Micchelli (Washington University, St. Louis, MO); Par6Δ226 FRT19A and UAS-aPKCCAAX were provided by C. Doe (University of Oregon, Eugene, OR); Sec5E10 FRT40A and UAS-Sec5 were provided by T. L. Schwarz (Harvard Medical School, Boston, MA); dRip11d00190 was provided by the Exelixis Collection (Harvard Medical School); and UAS-Rho1 RNAi, UAS-Cdc42 RNAi, and UAS-Cdc42 were generated by our lab (Warner and Longmore, 2009a (link), b (link)).
Protocol detail
Drosophila Endocytic Trafficking Regulators
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Variable analysis
independent variables
- UAS-YFP-Rab11
- UAS-YFP-Rab11^Q70L
- UAS-YFP-Rab11^S25N
- UAS-Rab11-RNAi
- UAS-Cdc42^V12
- UAS-YFP-Rab8^Q67L
- UAS-YFP-Rab4
- UAS-YFP-Rab14^Q97L
- UAS-aPKC-RNAi
- UAS-Rho1
- Dia^5 FRT40A
- UAS-YFP-Rab5
- Nuf^EY11342
- Rho1^72F
- Cdc42^4 FRT19A
- UAS-Sec8
- Rab11^EP3017
- UAS-Rok-CAT
- UAS-LIMK-HA
- UAS-MBS-RNAi
- Sqh^AX3; RLC-GFP
- UAS-Rab5-RNAi
- UAS-Rok-RNAi
- UAS-PKN-RNAi
- Shg^R69 FRT42D
- Par6^Δ226 FRT19A
- UAS-aPKC^CAAX
- Sec5^E10 FRT40A
- UAS-Sec5
- DRip11^d00190
- UAS-Rho1 RNAi
- UAS-Cdc42 RNAi
- UAS-Cdc42
- Not explicitly mentioned
- All crosses and staging were performed at 25°C
- Not explicitly mentioned
- Not explicitly mentioned
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