Biomarkers Processing and Analysis

Blood samples were collected in 10-ml EDTA tubes and immediately transferred to our laboratory where they were centrifuged and aliquoted within 2 h after collection. CSF samples were collected on the same day of blood extraction and processed in polypropylene tubes following international recommendations [33 (link)]. All samples were processed and aliquoted within the first two hours after lumbar puncture. The plasma and CSF aliquots were stored at − 80 °C until analysis. pGFAP and pNfL concentrations were measured using the SR-X single molecule array. CSF AD core biomarkers (Aβ42, Aβ40, tTau and pTau181) were measured in the fully-automated platform Lumipulse (Fujirebio-Europe), and levels of CSF NfL (Uman Diagnostics) and CSF YKL-40 (MicroVue™, Quidel) were measured by ELISA according to previously reported methods [7 (link), 33 (link)]. All samples and calibration curves were measured in duplicate. Intra- and inter-assay coefficients of variations were 5.3% and 9.6%, respectively. The pre-analytical processing protocol for blood and CSF in the SPIN cohort has been described in detail [28 (link), 29 (link)].

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Zhu N., Santos-Santos M., Illán-Gala I., Montal V., Estellés T., Barroeta I., Altuna M., Arranz J., Muñoz L., Belbin O., Sala I., Sánchez-Saudinós M.B., Subirana A., Videla L., Pegueroles J., Blesa R., Clarimón J., Carmona-Iragui M., Fortea J., Lleó A, & Alcolea D. (2021). Plasma glial fibrillary acidic protein and neurofilament light chain for the diagnostic and prognostic evaluation of frontotemporal dementia. Translational Neurodegeneration, 10, 50.

Publication 2021

Assay Biomarkers Blood Diagnostics Edta Elisa Lumbar puncture Plasma Polypropylene Ykl 40

Corresponding Organization :

Other organizations : Hospital de Sant Pau, Universitat Autònoma de Barcelona, Biomedical Research Networking Center on Neurodegenerative Diseases

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

PGFAP concentrations
PNfL concentrations
CSF Aβ42
CSF Aβ40
CSF tTau
CSF pTau181
CSF NfL
CSF YKL-40

control variables

Blood samples were collected in 10-ml EDTA tubes
Blood samples were immediately transferred to the laboratory
Blood samples were centrifuged and aliquoted within 2 h after collection
CSF samples were collected on the same day of blood extraction
CSF samples were processed in polypropylene tubes following international recommendations
All samples were processed and aliquoted within the first two hours after lumbar puncture
The plasma and CSF aliquots were stored at − 80 °C until analysis
All samples and calibration curves were measured in duplicate
Intra- and inter-assay coefficients of variations were 5.3% and 9.6%, respectively

positive controls

Calibration curves were measured in duplicate

negative controls

None explicitly mentioned

Annotations

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