Mitochondrial Genome Sequencing and Assembly

Genome sequencing was performed using HiSeqX at Macrogen Inc., Seoul, Korea from extracted DNA of the WBPH sample. De novo assembly and confirmation were done by Velvet v1.2.10 [21 (link)] after filtering raw reads using Trimmomatic v0.33 [47 (link)] with default parameters. The assembled mitochondrial genome sequence was confirmed with BWA v0.7.17 [48 ] (alignment of raw reads against the assembled sequence) and SAMtools v1.9 (tview mode for a manual check of each base) [49 (link)]. All bioinformatic processes were conducted under the environment of the Genome Information System (GeIS; http://geis.infoboss.co.kr/; 24 November 2021) which have been utilized in the various genomic studies [50 (link),51 (link),52 (link),53 ].

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Park J., Xi H., Park J, & Seo B.Y. (2021). A New Mitochondrial Genome of Sogatella furcifera (Horváth) (Hemiptera: Delphacidae) and Mitogenome-Wide Investigation on Polymorphisms. Insects, 12(12), 1066.

Publication 2021

HiSeqX

Genomic Mitochondrial genome

Corresponding Organization : National Institute of Agricultural Science and Technology

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Variable analysis

independent variables

Genome sequencing method (HiSeqX)
De novo assembly method (Velvet v1.2.10)
Read filtering method (Trimmomatic v0.33)

dependent variables

Assembled mitochondrial genome sequence

control variables

Alignment of raw reads against the assembled sequence (BWA v0.7.17)
Manual check of each base (SAMtools v1.9 tview mode)

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