Heparin and no heparin microgel populations were mixed to yield a 10% heparin scaffold mixture (e.g., 10 µL of heparin microgels combined with 90 µL of no heparin gels). The MAP scaffold mixture was then mixed 1:1 with 40 µm Eosin-Y in PBS and allowed to incubate for at least 10 min. Next, the microgels were centrifuged at 4696 g for 5 min to pellet the gel, and the photoinitiator solution was removed. Microgels were then loaded in 1 mL syringes which could be used in surgeries. All preparation was performed in a biosafety cabinet.
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