Interphase nuclei were prepared from infected 855-19 T-cells 3 dpi (lytic) and 14 dpi (latent), as described previously [42 (link)]. Briefly, MDV genomes were detected using a set of PCR-based MDV probes and visualized using Cy3 Streptavidin (1:1000 dilution; GE Healthcare, Munich, Germany) [43 (link)]. Images of interphases were taken using Axio Imager M1 (Zeiss) and analyzed with the ImageJ software.
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