Detecting Latent and Lytic Herpesvirus
Corresponding Organization : Freie Universität Berlin
Other organizations : Humboldt-Universität zu Berlin
Variable analysis
- Time point of sample collection (3 dpi and 14 dpi)
- Presence and visualization of MDV genomes in interphase nuclei
- Preparation of interphase nuclei from 855-19 T-cells
- Use of PCR-based MDV probes to detect MDV genomes
- Visualization of MDV genomes using Cy3 Streptavidin
- Imaging of interphase nuclei using Axio Imager M1 (Zeiss)
- Analysis of images using ImageJ software
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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