Seven week old male normotensive Wistar-Kyoto (WK) and spontaneously hypertensive (SHR) rats were randomly assigned either to the sedentary group (SHRsed; n=10 and WKsed; n=10) or to the exercise group (SHRex; n=10 and WKex; n=10). Exercise groups were subjected to moderate-intensity exercise on a motor-driven treadmill continuously for a period of 16 weeks. Animals were euthanized twenty-four hours after the last exercise session at the age of 23 weeks and left ventricle (LV) tissues were collected for later analyses. We performed the following experimental procedures: blood pressure measurements, echocardiographic analysis, real time RT-PCR, western blot analysis, electron paramagnetic resonance (EPR) studies, antioxidant assays, electrophoretic mobility shift assay (EMSA), reverse-phase high-performance liquid chromatography (HPLC), ELISA, and statistical analysis.
Protocol detail
Exercise and Hypertension in Rats
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Animals
Antioxidant
Assays
Blood pressure measurements
Echocardiographic
Electron paramagnetic resonance
Electrophoretic mobility shift assay
Elisa
Left ventricle
Male
Real time pcr
Reverse phase liquid chromatography
Spontaneously hypertensive rats
Tissues
Western blot analysis
Wistar kyoto
Corresponding Organization :
Other organizations : Louisiana State University
Top 5 similar protocols
Protocol cited in 11 other protocols
Variable analysis
independent variables
- Exercise group (SHRex and WKex)
- Blood pressure measurements
- Echocardiographic analysis
- Real time RT-PCR
- Western blot analysis
- Electron paramagnetic resonance (EPR) studies
- Antioxidant assays
- Electrophoretic mobility shift assay (EMSA)
- Reverse-phase high-performance liquid chromatography (HPLC)
- ELISA
- Sedentary group (SHRsed and WKsed)
- Seven week old male normotensive Wistar-Kyoto (WK) and spontaneously hypertensive (SHR) rats
- Age of 23 weeks
- No positive controls specified
- No negative controls specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!