STR Profiling and Sex Determination

STR loci and the amelogenin sex-determining marker were amplified using the COrDIS plus kit (Gordiz, Moscow, Russia) (detecting amelogenin, D5S818, D21S11, D7S820, CSF1PO, D2S1338, D3S1358, vWA, D8S1179, D16S539, TPOX, TH01, D19S433, D18S51, FGA, and D13S317) according to the manufacturer’s instructions in a GeneAmp^®PCR system 9700 (Thermo Fisher Scientific, Waltham, MA, USA). Electrophoretic analysis was performed using a 3730/3130xl DNA Analyzer (Thermo Fisher Scientific, Waltham, MA, USA). After electrophoresis, the data were analyzed with Gene Mapper^® ID-X Software v1.5 [22 (link)] (Thermo Fisher Scientific, Waltham, MA, USA) to categorize peaks by size in relation to an internal standard allelic ladder.

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Lomovskaya Y.V., Kobyakova M.I., Senotov A.S., Lomovsky A.I., Minaychev V.V., Fadeeva I.S., Shtatnova D.Y., Krasnov K.S., Zvyagina A.I., Akatov V.S, & Fadeev R.S. (2022). Macrophage-like THP-1 Cells Derived from High-Density Cell Culture Are Resistant to TRAIL-Induced Cell Death via Down-Regulation of Death-Receptors DR4 and DR5. Biomolecules, 12(2), 150.

Publication 2022

GeneAmp®PCR system 9700 Gene Mapper® ID-X Software v1.5

Allelic Amelogenin Electrophoresis Gene

Corresponding Organization : Institute of Theoretical and Experimental Biophysics

Other organizations : Pushchino State Institute of Natural Sciences

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Variable analysis

independent variables

COrDIS plus kit (Gordiz, Moscow, Russia) used for amplification of STR loci and the amelogenin sex-determining marker

dependent variables

Sizes of amplified STR loci and amelogenin peaks detected by electrophoretic analysis using a 3730/3130xl DNA Analyzer (Thermo Fisher Scientific, Waltham, MA, USA)

control variables

Manufacturer's instructions for the COrDIS plus kit (Gordiz, Moscow, Russia)
GeneAmp®PCR system 9700 (Thermo Fisher Scientific, Waltham, MA, USA) used for amplification
Gene Mapper® ID-X Software v1.5 [22 (link)] (Thermo Fisher Scientific, Waltham, MA, USA) used for data analysis

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