Primary antibodies used for indirect immunofluorescence were as follows: for Figures 1, 4, S5, and 6 we used rat anti-ATP7B (S. Lutsenko, Johns Hopkins School of Medicine); For Figures 2, S3, S4, 5, 7 & 8 we used rabbit anti-ATP7B (#ab124973, Abcam, Cambridge, MA); for Figures 3 and S1 we used rabbit anti-ATP7B (Dr. J. Gitlin, Brown Alpert Medical School, Providence, RI); rabbit anti-aminopeptidase N (APN, #1637, (22 (link)) guinea pig anti-dipeptidyl-peptidase 4 (DPP4, (41 (link))) goat anti-EEA1 (#sc-6415, Santa Cruz Biotechnology, Santa Cruz, CA); mouse anti-TGN38, and -Syntaxin 6 (BD Biosciences, San Jose, CA); mouse anti-LAMP1 (#H4A3-s, Developmental Studies Hybridoma Bank, Iowa City, IA); rabbit anti-Cathepsin D (Dr A. Hasilik, Marburg, Germany); rabbit anti-LC3B (#2775, Cell Signaling Technology, Danvers, MA). Secondary antibodies conjugated to Cy3 or Cy5 were from Jackson ImmunoResearch Laboratories (West Grove, PA), while those conjugated to Alexa 488, -568 or -647 were from Molecular Probes (Eugene, OR). Primary antibodies used for immunoblotting inculded: rabbit anti-ATP7B (#3985) (13 (link)), mouse anti-alpha tubulin, DM1A (Sigma, St. Louis, MO). Secondary antibodies conjugated to horseradish peroxidase (HRP) were from G.E. Healthcare (Buckinghamshire, United Kingdom).