Human brain microvascular endothelial cells (hBMECs, passages 3–6) were cultured as we previously described7 (link), 49 (link). In some experiments, cells were treated with empagliflozin (1 μM), adding glucose at 5 or 30 mM, for 48 h. The endothelial permeability assay was performed using fibronectin-coated trans-well filters (Corning Inc., Corning, NY, USA), as we previously reported7 (link), 49 (link), 50 (link).
Protocol detail
Assessing Endothelial Permeability in hBMECs
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Assay
Brain
Cells
Empagliflozin
Endothelial
Endothelial cells
Fibronectin
Glucose
Human
Permeability
Corresponding Organization : Albert Einstein College of Medicine
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Variable analysis
independent variables
- Empagliflozin (1 μM)
- Glucose concentration (5 mM or 30 mM)
- Endothelial permeability
- Human brain microvascular endothelial cells (hBMECs, passages 3-6)
- Fibronectin-coated trans-well filters
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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