The standard zebrafish chow Otohime B2 was obtained from Marubeni Nisshin Feed Co., Ltd. (Tokyo, Japan). Gluten was obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan), and α-potato starch, corn oil, and lard were purchased from Oriental Yeast Co., Ltd. (Tokyo, Japan).
GTE was prepared and analyzed as previously described [22 (link), 25 (link)]. The composition of catechins was measured by high-performance liquid chromatography. The total catechin content in the GTE was 79.6% to 81.8% (w/w), which comprised epigallocatechin gallate (accounting for 43.6%–44.4% of the total), epigallocatechin (20.4%–20.7%), epicatechin gallate (12.0%–12.3%), epicatechin (8.1%–8.3%), gallocatechin (6.9%–7.0%), gallocatechin gallate (4.4%), and others (3.7%–3.8%). The caffeine content was 0.0% to 0.1%.
GTE was prepared and analyzed as previously described [22 (link), 25 (link)]. The composition of catechins was measured by high-performance liquid chromatography. The total catechin content in the GTE was 79.6% to 81.8% (w/w), which comprised epigallocatechin gallate (accounting for 43.6%–44.4% of the total), epigallocatechin (20.4%–20.7%), epicatechin gallate (12.0%–12.3%), epicatechin (8.1%–8.3%), gallocatechin (6.9%–7.0%), gallocatechin gallate (4.4%), and others (3.7%–3.8%). The caffeine content was 0.0% to 0.1%.
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