Single Molecule Localization Microscopy

Single molecule localization was performed using an ImageJ ThunderStorm plugin64 (link). The default values were used for the analysis (B-spline wavelet filter—order 3 and scale 2.0, approximate localization by eight-neighbourhood local maximum, Subpixel localization by PSF integrated Gaussian with the weighted least squares fitting method with a 3 pixels fitting radius and 1.6 pixels initial sigma). Particle coordinates and statistical properties were exported and further analysis was conducted using Matlab 2013a (MathWorks). Image drift and vibrations were corrected by mean image displacement values using a custom code in MATLAB.

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Golan Y, & Sherman E. (2017). Resolving mixed mechanisms of protein subdiffusion at the T cell plasma membrane. Nature Communications, 8, 15851.

Publication 2017

Matlab 2013a

Radius Vibrations

Corresponding Organization :

Other organizations : Hebrew University of Jerusalem

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Variable analysis

independent variables

ImageJ ThunderStorm plugin settings (B-spline wavelet filter—order 3 and scale 2.0, approximate localization by eight-neighbourhood local maximum, Subpixel localization by PSF integrated Gaussian with the weighted least squares fitting method with a 3 pixels fitting radius and 1.6 pixels initial sigma)

dependent variables

Particle coordinates
Particle statistical properties

control variables

Default values used for the analysis

controls

No positive or negative controls were explicitly mentioned in the input.

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