Murine Lyme Disease Transmission Model

Female C3H/HeJ or NOD.Cg-Prkd^SCID/J (SCID) mice (The Jackson Laboratory) were inoculated with 1 × 10⁵ organisms via intradermal injection. 4-to-8 weeks after inoculation, animals were sacrificed, and blood and tissues were collected for serology and culturing, respectively. Pathogen-free Ixodes scapularis larvae were purchased from Oklahoma State University Tick Rearing Facility (Stillwater, Oklahoma, USA). Naive larvae were infected by immersion (95 (link)), fed to repletion on naive C3H/HeJ mice, and allowed to molt. Infected nymphs were fed on naive C3H/HeJ mice until fully engorged as previously described (13 (link), 96 (link)).

Free full text: Click here

Grassmann A.A., Tokarz R., Golino C., McLain M.A., Groshong A.M., Radolf J.D, & Caimano M.J. (2023). BosR and PlzA reciprocally regulate RpoS function to sustain Borrelia burgdorferi in ticks and mammals. The Journal of Clinical Investigation, 133(5), e166710.

Publication 2023

Animals Blood C3h mice Female Immersion Inoculation Intradermal injection Ixodes scapularis Larvae Molt Nymphs Pathogen Scid mice Tick Tissues

Corresponding Organization : Institute of Molecular Biology and Biophysics

Other organizations : Institute of Infection and Immunity, Columbia University

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Inoculation of C3H/HeJ or NOD.Cg-Prkd^SCID/J (SCID) mice with 1 × 10^5 organisms via intradermal injection

dependent variables

Serology and culturing of blood and tissues after 4-to-8 weeks of inoculation

control variables

Female C3H/HeJ or NOD.Cg-Prkd^SCID/J (SCID) mice used as experimental subjects
Pathogen-free Ixodes scapularis larvae from Oklahoma State University Tick Rearing Facility
Naive C3H/HeJ mice used for feeding infected nymphs

controls

Naive larvae infected by immersion and fed to repletion on naive C3H/HeJ mice
Infected nymphs fed on naive C3H/HeJ mice until fully engorged

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!