Comprehensive Protein Expression Analysis

Equal amounts of cell lysates were loaded for immunoprecipitation or immunoblotting using the indicated primary antibodies [37 (link)]. Primary antibodies used in this study were as follows: LC3, ATG-5, ATG-7, Caspase-3, Caspase-9 (Novus biological), PARP, p-Stat-3, Survivin, cleaved Caspase-3 and Bcl-2 (Cell Signaling Technology), Hif1α, Beclin-1, Bim, Bax (BD Bioscience), Bnip3 (Abcam), Noxa, LIVIN (Imgenex), PON2 (Abnova), β-actin (Sigma-Aldrich). Mcl-1, Bak, Bcl-x_L (Santa Cruz). Anti-Bak (Ab-1) were developed against recombinant human Bak from which the C-terminal and transmembrane domains were deleted (Calbiochem).

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Maji S., Samal S.K., Pattanaik L., Panda S., Quinn B.A., Das S.K., Sarkar D., Pellecchia M., Fisher P.B, & Dash R. (2015). Mcl-1 is an important therapeutic target for oral squamous cell carcinomas. Oncotarget, 6(18), 16623-16637.

Publication 2015

Caspase-3 Caspase-9 p-Stat-3 Survivin cleaved caspase-3 Bcl-2 Hif1α Beclin-1 Bax Bnip3 β-actin Mcl-1 Bcl-xL

Actin Antibodies Bcl 2 Beclin 1 Biological Caspase 3 Caspase 9 Cell Hif1α Human Immunoprecipitation Livin Novus Pon2 Survivin

Corresponding Organization :

Other organizations : Institute of Life Sciences, Manipal Academy of Higher Education, Siksha O Anusandhan University, Virginia Commonwealth University, Sanford Burnham Prebys Medical Discovery Institute

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Variable analysis

independent variables

Cell lysates loaded for immunoprecipitation or immunoblotting

dependent variables

Protein expression levels of: LC3, ATG-5, ATG-7, Caspase-3, Caspase-9, PARP, p-Stat-3, Survivin, cleaved caspase-3, Bcl-2, Hif1α, Beclin-1, Bim, Bax, Bnip3, Noxa, LIVIN, PON2, Mcl-1, Bak, Bcl-xL

control variables

Equal amounts of cell lysates loaded for immunoprecipitation or immunoblotting

controls

Primary antibodies used as positive controls: LC3, ATG-5, ATG-7, Caspase-3, Caspase-9, PARP, p-Stat-3, Survivin, cleaved caspase-3, Bcl-2, Hif1α, Beclin-1, Bim, Bax, Bnip3, Noxa, LIVIN, PON2, Mcl-1, Bak, Bcl-xL, β-actin

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