Protein Expression Analysis of DP T Thymocytes

Total protein extracts were prepared from sorted DP T thymocyte samples and Western blotting analysis was conducted, as previously described (43 (link)), using anti-p21 antibody (C-19, sc-397, Santa Cruz), and anti-Caspase-3 or anti-cleaved Caspase-3 antibody (#9665 and #9661, respectively, both from Cell signaling). Anti-β-actin antibody (Sigma-Aldrich), was used to normalize protein expression levels. Densitometric analysis was performed using ImageStudio software (LI-COR Biosciences).

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Grazioli P., Orlando A., Giordano N., Noce C., Peruzzi G., Scafetta G., Screpanti I, & Campese A.F. (2020). NF-κB1 Regulates Immune Environment and Outcome of Notch-Dependent T-Cell Acute Lymphoblastic Leukemia. Frontiers in Immunology, 11, 541.

Publication 2020

anti-Caspase-3 anti-cleaved Caspase-3 antibody Anti-β-actin antibody ImageStudio software

Actin Anti antibody Caspase 3 Densitometric Protein Thymocyte Western blotting analysis

Corresponding Organization : Sapienza University of Rome

Other organizations : Italian Institute of Technology

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Variable analysis

independent variables

Sorted DP T thymocyte samples

dependent variables

Protein expression levels of p21
Protein expression levels of Caspase-3 and cleaved Caspase-3

control variables

Anti-β-actin antibody used to normalize protein expression levels

controls

Positive control: None specified
Negative control: None specified

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