Extraction and Characterization of Plantago Ovata Mucilage

CMC, glycerol (GLY), AMK, NaOH, HCl, penicillin, streptomycin, Mueller–Hinton (MH) agar, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) solution, PBS tablets, silica gel beads, and transdermal diffusion membranes were obtained from Millipore Sigma (St. Louis, MO, US) and used without any modification. The human fetal lung fibroblast cells (MRC-5) (ATCC CCL-171) and bacterial strains (Escherichia coli (ATCC 8739), Pseudomonas aeruginosa (ATCC 9027), and Staphylococcus aureus (ATCC 25923)) were procured from the American Type Culture Collection (ATCC, Manassas, VI, USA). The culture medium RPM1-1640 and fetal bovine serum (FBS) were obtained from Nacalai Tesque (Kyoto, Japan) and Biosera (Nuaille, France), respectively. All experiments were performed in double-distilled water (DDW) unless otherwise specified. AX was extracted and characterized from Plantago ovata/psyllium seed husk (PSH) using our previously described method [12 (link),29 (link)].

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Alruwaili N.K., Ahmad N., Alzarea A.I., Alomar F.A., Alquraini A., Akhtar S., Shahari M.S., Zafar A., Elmowafy M., Elkomy M.H., Dolzhenko A.V, & Iqbal M.S. (2022). Arabinoxylan-Carboxymethylcellulose Composite Films for Antibiotic Delivery to Infected Wounds. Polymers, 14(9), 1769.

Publication 2022

NaOH HCl penicillin streptomycin PBS tablets transdermal diffusion membranes MRC-5) (ATCC CCL-171) Staphylococcus aureus (ATCC 25923 fetal bovine serum (FBS)

Agar Bacterial Bromide Culture medium Diffusion Escherichia coli Fetal Fetal bovine serum Fibroblast cells Glycerol Human Lung Membranes Penicillin Plantago ovata Pseudomonas aeruginosa Psyllium seed husk Silica gel Staphylococcus aureus Strains Streptomycin

Corresponding Organization : Al Jouf University

Other organizations : Imam Abdulrahman Bin Faisal University, Al Baha University, Monash University Malaysia, Forman Christian College

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Variable analysis

independent variables

Glycerol (GLY)
Penicillin
Streptomycin
AX extracted and characterized from Plantago ovata/psyllium seed husk (PSH)

dependent variables

Cell viability and proliferation of human fetal lung fibroblast cells (MRC-5)
Antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus

control variables

Mueller–Hinton (MH) agar
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) solution
PBS tablets
Silica gel beads
Transdermal diffusion membranes
RPM1-1640 culture medium
Fetal bovine serum (FBS)
Double-distilled water (DDW)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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