Synthesis and Characterization of Lactoferrampin Peptides

The LF-1 (WKLLRKAWKLLRKA), LF-2 (GKLIWKLLRKAWKLLRKA), template (WKLLRKA), and parent lactoferrampin (WKLLSKAQEKFGKNKSR) peptides were synthesised using standard 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase protocols [21 (link)] and verified by mass spectrometry (3200 Q TRAP, Thermo Fisher Scientific, Waltham, MA, USA). Peptides were then purified by reverse-phase high-performance liquid chromatography (RP-HPLC; Thermo Fisher Scientific) to > 95% and stored at −20°C until use.
The physicochemical properties of peptides were analysed according to the following parameters [22 (link)]: hydrophobic moment (μH) and helical-wheel diagrams were determined at Heliquest (https://heliquest.ipmc.cnrs.fr/); molecular weight (MW) and net charge at pH 7 were calculated using the Peptide Property Calculator (http://www.pepcalc.com/ppc.php); and grand average hydropathy (GRAVY) was determined using the GRAVY Calculator (www.gravy-calculator.de).

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Luo J., Feng Z., Jiang W., Jiang X., Chen Y., Lv X, & Zhang L. (2021). Novel lactotransferrin-derived synthetic peptides suppress cariogenic bacteria in vitro and arrest dental caries in vivo: [Novel lactotransferrin-derived anticaries peptides]. Journal of Oral Microbiology, 13(1), 1943999.

Publication 2021

RP-HPLC

9 fluorenylmethoxycarbonyl Helical Hplc Lactoferrampin Mass spectrometry Parent Peptide

Corresponding Organization : Sichuan University

Other organizations : Sun Yat-sen University, Stomatology Hospital

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Variable analysis

independent variables

LF-1 (WKLLRKAWKLLRKA)
LF-2 (GKLIWKLLRKAWKLLRKA)
Template (WKLLRKA)
Parent lactoferrampin (WKLLSKAQEKFGKNKSR)

dependent variables

Physicochemical properties of peptides
Hydrophobic moment (μH)
Molecular weight (MW)
Net charge at pH 7
Grand average hydropathy (GRAVY)

control variables

Peptides were synthesized using standard 9-fluorenylmethoxycarbonyl (Fmoc) solid-phase protocols
Peptides were purified by reverse-phase high-performance liquid chromatography (RP-HPLC) to > 95%
Peptides were stored at −20°C until use

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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