High-throughput sequencing of lncRNA

The lncRNA expression in d5 and d10 cells were analyzed through high-throughput sequencing (Decode Genomics, China). In brief, total RNA was extracted from cells through Trizol reagent, and the RNA concentration, purity, and integrity were assessed and adjusted. Then, ribosomal RNA (rRNA) was removed by rRNA Removal Kit (Epicentre), and the remaining RNA was proposed to generate a sequencing library according to the methods as previously described [13 (link)]. Following, the library was sequenced on Illumina Hiseq X10 platform (Illumina) and the expression of lncRNAs was analyzed by Feature Counts.