RNA Extraction and Real-Time PCR Analysis
Corresponding Organization : Shinshu University
Other organizations : The University of Tokyo, Japan Science and Technology Agency, Okayama University
Variable analysis
- Plant genotype (O/E and WT)
- Expression levels of OsHKT1;4 and OsSMT3 genes
- RNA extraction method (RNeasy Plant Mini Kit)
- Reverse transcription and real-time PCR methods (PrimeScript RT Master Mix and Thermal Cycler Dice Real Time System II TP800)
- No positive or negative controls were explicitly mentioned.
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