Total RNA was extracted from each tissue of O/E and WT plants using an RNeasy Plant Mini Kit (Qiagen, Limburg, The Netherlands). Reverse transcription reactions and subsequent real time PCR analyses were performed using PrimeScriptâ„¢ RT Master Mix and a Thermal Cycler Dice Real Time System II TP800 (Takara, Japan), as described previously [23 (link)]. Primer sequences for OsHKT1;4 and an internal control OsSMT3 were shown in Table S1.
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