Lateral Flow Biosensor Platform for Rapid SARS-CoV-2 Detection

The LFB platform was prepared according to a previous report (Cheng et al., 2019 (link)). Briefly, the LFB contained four components: an absorbent pad, NC membrane, sample pad, and conjugate pad (Jie-Yi Biotechnology. Co., Ltd.). The components were assembled orderly on a backing card. The capture reagents, including anti-FAM, anti-Dig, and biotin-BSA (Abcam. Co., Ltd.), were immobilized by physical adsorption on the reaction regions. Then, anti-FAM was immobilized at test line 1 (TL1) (RdRp), and anti-Dig was immobilized at test line 2 (TL2) (N), while biotin-BSA was immobilized at the control line (CL); each line was separated by 5 mm. SA-PNPs (dye streptavidin-coated polymer nanoparticles) were gathered on the conjugate pad. The prepared biosensors were preserved in a plastic box with a desiccant gel at room temperature before use.