Western blotting was performed and immunoprecipitation according to our previous work [31 (link)]. For immunoprecipitation, rabbit polyclonal antibodies (Abcam) against BAF53a or Tip60 were used. The cytosolic fraction (2 mg/ml) from subcellular fractionation assay was used as input (IN). As negative control, no antibody was added to a same amount of IN and beads (Santa Cruz Biotechnology).
Free full text: Click here