To generate olaparib-resistant cells, C1 cells were treated with 0.75 mM MMS for 1 h and then continuously cultured in the presence of 1–20 μM olaparib for approximately 5 months. Olaparib-resistant clones were maintained in MEM supplemented with 10% FBS, 1% non-essential amino acids, 1% penicillin-streptomycin, and 1 μM olaparib. An isolated single clone#(2–4)9 was designated as C1/OLA cells.
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