Lipogenesis Inhibitors SREBP1, FASN, and ACC

The SREBP1 inhibitors, PF429242 and betulin, were purchased from Sigma Aldrich (St. Louis, MO) and Merck (Kenilworth, NJ), respectively. SREBP1 antibody was purchased from BD Biosciences (#557036; San Jose, CA). FASN antibody was purchased from Santa Cruz Biotechnology (SC-55580; Santa Cruz, CA). ACC antibody was purchased from Cell Signaling Technology, Inc (#3662; Beverly, MA). Nile Red (N1142), DAPI (62248) and the secondary antibodies, Alexa Fluor 488-Donkey anti-mouse (A32766) and Alexa Fluor 568-Donkey anti-rabbit (A10042) were purchased from Thermo Fisher Scientific (Waltham, MA). Other reagents and antibodies were the same as described previously^{13 (link), 23 (link)}.

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Chen Z., Yu D., Owonikoko T.K., Ramalingam S.S, & Sun S.Y. (2021). Induction of SREBP1 degradation coupled with suppression of SREBP1-mediated lipogenesis impacts the response of EGFR mutant NSCLC cells to osimertinib. Oncogene, 40(49), 6653-6665.

Publication 2021

PF429242 betulin SREBP1 antibody FASN antibody ACC antibody Alexa Fluor 488-Donkey anti-mouse (A32766)

Alexa 568 Alexa fluor 488 Antibodies Antibody Betulin Dapi Donkey Fasn Inhibitors Mouse Rabbit Srebp1

Corresponding Organization :

Other organizations : Emory University, UPMC Hillman Cancer Center

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Variable analysis

independent variables

PF429242
Betulin

dependent variables

SREBP1 levels
FASN levels
ACC levels

control variables

Cell line(s) used
Culture conditions
Time points

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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