Slides scanned at 20× magnification with Aperio ScanScope XT (Leica) were analyzed using Aperio ImageScope Positive Pixel Count software (PPC) (Leica). Parameters to quantify lipid droplet tissue percentage from H&E-stained slides, FOXA1 immunohistochemistry levels, and percent phospho-T156-FOXA2 (p-FOXA2)-positive nuclei are described in Supplementary Tables S3–5 . Stain levels from images of immunohistochemically stained tissue sections taken at 10× magnification were measured with ImageJ software (National Institutes of Health) using the Immunohistochemistry Image Analysis Toolbox(16 (link)) default H-DAB settings (imagej.nih.gov/ij/plugins/immunohistochemistry-toolbox/index.html ) to detect brown staining, then converting the brown stain image to 32-bit grayscale. Quantification parameters are listed in Supplementary Table S6 .
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Quantifying Lipid Droplets and Transcription Factors
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Corresponding Organization : Hong Kong Polytechnic University
Other organizations : Pacific Northwest Diabetes Research Institute, Mayo Clinic, University of Iowa
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Variable analysis
independent variables
- Magnification (20×)
- Lipid droplet tissue percentage
- FOXA1 immunohistochemistry levels
- Percent phospho-T156-FOXA2 (p-FOXA2)-positive nuclei
- H&E-stained slides
- Immunohistochemically stained tissue sections
- No positive or negative controls were explicitly mentioned in the provided information.
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