Non-Invasive Thermal Imaging Protocol

For non-invasive measurement of heat dissipation, infrared images of the dorsal and ventral sides were taken using a thermographic camera (Testo 875-1i, Testo AG, Lenzkirch, Germany) from 15 animals (vehicle-treated: n=7; T4-treated: n=8). The dorsal and ventral sides were defined as the surface excluding the head, neck, extremities, and tail. The shaved areas where the incisions were made for implantation of the osmotic pump and temperature logger were excluded from the analysis. Infrared images were generated and analyzed using IRsoft software (vers. 4.7, Testo).

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Gerhardt P., Begall S., Frädrich C., Renko K., Heinrich A., Köhrle J, & Henning Y. (2024). Low thyroxine serves as an upstream regulator of ecophysiological adaptations in Ansell’s mole-rats. Frontiers in Endocrinology, 15, 1329083.

Publication 2024

Corresponding Organization :

Other organizations : University of Duisburg-Essen, Humboldt-Universität zu Berlin, Charité - Universitätsmedizin Berlin, Freie Universität Berlin, Federal Institute for Risk Assessment

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Variable analysis

independent variables

Treatment (vehicle vs. T4)

dependent variables

Heat dissipation (measured through infrared imaging of the dorsal and ventral sides)

control variables

Shaved areas where incisions were made for implantation of the osmotic pump and temperature logger (excluded from the analysis)
Head, neck, extremities, and tail (excluded from the dorsal and ventral side measurements)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

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