According to the related reference [35 (link)], the samples were taken at the specific nodes and serum samples were prepared. Above 3 mL, venous blood was gotten from specific rabbits. The blood samples were centrifuged for 10 min (4 °C, 3500 r/min) to collect the serum sample. On the analyzing day, after serum sample thawed, vortex 3 min by using Vortex Mixer XW-80A. Then, 100 μL serum sample was taken for metabolomics analysis. The proteins in sample were removed by using 400 μL acetonitrile. The supernatant was evaporated to dryness by Centrivap Concentrator (LABCONCO, USA). The residue was reconstituted in the initial mobile phase, vortex 3 min, and ultrasonic extracted 5 min at 4 °C. Through the centrifugation, supernatant was transferred into 150 μL glass insert in a 1.5 mL amber glass vial and analyzed.
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