Protein Expression Analysis in Skin

The protein levels of KRT5, KRT14, KRT1, KRT10, STAT1, and S100A7 were examined by immunoblotting following the methods described before^{34 (link)} with primary antibodies against KRT5 (ab52635; Abcam, Cambridge, MA, USA), KRT14 (ab7800, Abcam), KRT1 (ab93652, Abcam), KRT10 (ab76318, Abcam), STAT1 (ab109320, Abcam), and S100A7 (ab13680, Abcam) and the HRP-conjugated secondary antibody. Signals were visualized using enhanced chemiluminescent (ECL) substrates (Millipore, MA, USA) with normalization to GAPDH.

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Luo M., Huang P., Pan Y., Zhu Z., Zhou R., Yang Z, & Wang C. (2021). Weighted gene coexpression network and experimental analyses identify lncRNA SPRR2C as a regulator of the IL-22-stimulated HaCaT cell phenotype through the miR-330/STAT1/S100A7 axis. Cell Death & Disease, 12(1), 86.

Publication 2021

ab52635 ab7800 ab93652 ab76318 ab109320 ab13680 ECL) substrates

Antibodies Antibody Gapdh Krt1 Krt10 Krt14 Krt5 Protein S100a7 Stat1

Corresponding Organization :

Other organizations : Hunan University, Hunan University of Traditional Chinese Medicine

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Variable analysis

independent variables

Primary antibodies against KRT5, KRT14, KRT1, KRT10, STAT1, and S100A7

dependent variables

Protein levels of KRT5, KRT14, KRT1, KRT10, STAT1, and S100A7

control variables

GAPDH (used for normalization)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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