HCT116 and HT29 were cultured in RPMI-1640 medium containing 10% fetal bovine serum (Thermo Fisher, Waltham, MA, USA). To induce oxaliplatin resistance, HCT116 and HT29 were treated with 0.1 μM oxaliplatin (Sigma-Aldrich, St. Louis, MO, USA) for 9 days, and then incubated with 0.5 μM oxaliplatin for 15 days. Followed by cultured in medium containing 1 μM oxaliplatin for 30 days, the cells were exposed to 2 μM oxaliplatin to establish oxaliplatin-resistant CRC cells (HCT116/R and HT29/R) according to previous study [15 (link)]. To induce inactivation of MAPK signaling, cells were treated with 20 nM SB 203580 (Thermo Fisher) for 24 hours.
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Establishing Oxaliplatin-Resistant Colorectal Cancer Cells
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Corresponding Organization : Ningbo University
Other organizations : Ningbo No.6 Hospital
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Variable analysis
independent variables
- Oxaliplatin treatment concentration
- Duration of oxaliplatin treatment
- SB 203580 treatment (MAPK signaling inhibitor)
- Establishment of oxaliplatin-resistant colorectal cancer (CRC) cell lines (HCT116/R and HT29/R)
- Cell lines used: HCT116 and HT29
- Culture medium: RPMI-1640 with 10% fetal bovine serum
- Oxaliplatin source: Sigma-Aldrich
- SB 203580 source: Thermo Fisher
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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