Peptide Identification and Protein Interaction Analysis

The reading and identification of the peptides were conducted by a nanoAcquity UPLCXevo QTof MS system (Waters, Manchester, UK), using the Protein Lynx Global Server (PLGS), as previously detailed [31 (link)]. The proteins verification was determined by downloading the Uniprot database. Then, the bioinformatics analyses were performed using Cytoscape (3.6.1 version, Java®) with the ClueGO plugin for the determination of biological process groups, based on Gene Ontology (GO) annotations [32 (link)]. Additionally, the ClusterMarker plugin was for the protein-interaction network.

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Cunha Nascimento P., Alana Bragança Aragão W., Oliveira Bittencourt L., Dionizio A., A. R. Buzalaf M., Crespo-Lopez M.E, & Lima R.R. (2021). Maternal methylmercury exposure changes the proteomic profile of the offspring’s salivary glands: Prospects on translational toxicology. PLoS ONE, 16(11), e0258969.

Publication 2021

nanoAcquity UPLCXevo QTof MS system

Biological determination Lynx Peptides Proteins

Corresponding Organization : Universidade Federal do Pará

Other organizations : Universidade de São Paulo

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Identification of peptides
Verification of proteins

control variables

None explicitly mentioned

controls

Positive control: Uniprot database used for protein verification
Negative control: Not specified

Annotations

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