Western Blot Analysis of Protein Targets

Western analysis was performed as described previously (15 (link), 16 (link)). Target proteins were detected by using specific antibodies against BPTF (A300-973A at 1:1000 dilution) (Bethyl Laboratories, Montgomery, TX), pAKT (Ser473, #9271 at 1:500 dilution), total AKT (#4685, at 1:500 dilution), pGSK-3β (Ser9, #9323 at 1:500 dilution), CCND1 (#2978 at 1:500), BCL2 (#15071 at 1:500) (Cell Signaling Technology, Danvers, MA) and GAPDH (AB2302 at 1:10000 dilution, EMD Millipore, Billerica, MA).

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Bezrookove V., Khan I.A., Nosrati M., Miller JR I.I.I., McAllister S., Dar A.A, & Kashani-Sabet M. (2022). BPTF promotes the progression of distinct subtypes of breast cancer and is a therapeutic target. Frontiers in Oncology, 12, 1011173.

Publication 2022

pAKT pGSK-3β CCND1 BCL2 AB2302

Antibodies Bcl2 Ccnd1 Dilution Gapdh Proteins

Corresponding Organization :

Other organizations : California Pacific Medical Center

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of target proteins detected by specific antibodies:
- BPTF
- pAKT (Ser473)
- Total AKT
- pGSK-3β (Ser9)
- CCND1
- BCL2
- GAPDH

control variables

None explicitly mentioned

controls

Positive controls: None explicitly mentioned
Negative controls: None explicitly mentioned

Annotations

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