Screen-printed carbon electrodes (SPCEs) were custom-made from Botan Technology Co., Ltd. (Weihai, China). Potassium ferricyanide (K3[Fe(CN)6]) and potassium ferrocyanide (K4[Fe(CN)6]·3H2O) were purchased from Guangfu Technology Development Co., Ltd. (Tianjin, China). Potassium chloride (KCl), sodium dihydrogen phosphate (NaH2PO4·2H2O) and disodium hydrogen phosphate (Na2HPO4·12H2O) were purchased from Sinopharm Chemical Reagent Beijing Co., Ltd. (Beijing, China). Graphene (GR) and Au nanoparticles (AuNPs) were purchased from Jiangsu Xianfeng Nanomaterials Technology Co., Ltd. (Nanjing, China). Nafion (10 wt% in H2O) was purchased from Maclean Biochemical Technology Co., Ltd. (Shanghai, China). Acetylcholinesterase (AChE, 2 KU), acetylthiocholine chloride (ATCl), isocarbophos (ICP) were purchased from Sigma Aldrich (USA). All other chemicals and reagents used in this study were of analytical grade, and all aqueous solutions were prepared with ultrapure water (18.25 MΩ cm−1) by a Millipore Direct-Q water system.
Protocol detail
Electrochemical Biosensor for Insecticide Detection
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Acetylcholinesterase
Acetylthiocholine
Ache
Carbon
Chloride
Disodium hydrogen phosphate
Graphene
Isocarbophos
Nafion
Potassium ferricyanide
Potassium ferrocyanide
Sodium dihydrogen phosphate
Corresponding Organization : National University of Defense Technology
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Variable analysis
independent variables
- Screen-printed carbon electrodes (SPCEs)
- Graphene (GR)
- Au nanoparticles (AuNPs)
- Nafion
- Acetylcholinesterase (AChE)
- Acetylthiocholine chloride (ATCl)
- Isocarbophos (ICP)
- Measured outcomes (not explicitly mentioned)
- Potassium ferricyanide (K3[Fe(CN)6])
- Potassium ferrocyanide (K4[Fe(CN)6]·3H2O)
- Potassium chloride (KCl)
- Sodium dihydrogen phosphate (NaH2PO4·2H2O)
- Disodium hydrogen phosphate (Na2HPO4·12H2O)
- Ultrapure water (18.25 MΩ cm-1)
- Positive control: Not specified
- Negative control: Not specified
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