The antibodies used were LDH (Santa Cruz, sc-133123), Histone H3 (Abcam, ab1791) Actinin (Santa Cruz, sc-17829), Streptavidin-HRP (Cell Signaling 3999S), eIF3A (Abcam, ab118357), eIF4A1 (Abcam, ab31217), FXR1 (Abcam, ab129089), PABP (Abcam, ab21060), eIF5A (Abcam, ab32443), eIF2α (Invitrogen, AH00802), eIF4G1 (Abcam, ab2609), P53 (Santa-Cruz, sc-126 X), eIF4A3 (Abcam, ab32485), eIF4E (Cell Signaling, 20675), RPS6 (Abcam, ab58350), eIF3D (Abcam, ab12442), S6K1 (Abcam, ab32529), S6K1pT389 (Abcam, ab2571), RPS6pS235 (Abcam, ab12864), KDM5A (Abcam, ab70892). KDM4A and β-Actin antibodies were described in (23 (link)). MCM7 antibody was described in (8 (link)). KDM4A immunoprecipitations were performed with KDM4A-P006.
Protocol detail
Antibody-based Protein Interaction Analysis
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Corresponding Organization :
Other organizations : Massachusetts General Hospital, Harvard University, Structural Genomics Consortium, University of Toronto
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Variable analysis
independent variables
- KDM4A immunoprecipitations were performed with KDM4A-P006
- Histone H3
- Actinin
- Streptavidin-HRP
- EIF3A
- EIF4A1
- EIF5A
- EIF2α
- EIF4G1
- EIF4A3
- EIF4E
- EIF3D
- S6K1pT389
- RPS6pS235
- KDM5A
- KDM4A
- β-Actin
- β-Actin
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