Assessing Astrocytoma Cell Viability, Proliferation, and Invasion
The CCK8 (Bimake) and EDU incorporation assays (Ribobio) were performed according to the manufacturers’ procedures. Cell viability was assessed by a CCK8 assay as previously described (25 (link)). The proliferation ability of astrocytoma cells was detected by an EDU incorporation assay as previously described (26 (link)). The invasive ability of astrocytoma cells was tested by a transwell assay (Corning Inc.) as previously described (25 (link)).
Zhang Y., Feng J., Fu H., Liu C., Yu Z., Sun Y., She X., Li P., Zhao C., Liu Y., Liu T., Liu Q., Liu Q., Li G, & Wu M. (2018). Coagulation Factor X Regulated by CASC2c Recruited Macrophages and Induced M2 Polarization in Glioblastoma Multiforme. Frontiers in Immunology, 9, 1557.
Treatment with CCK8 (Bimake) and EDU incorporation assays (Ribobio)
dependent variables
Cell viability assessed by CCK8 assay
Proliferation ability of astrocytoma cells detected by EDU incorporation assay
Invasive ability of astrocytoma cells tested by transwell assay
control variables
Procedures followed according to manufacturers' instructions
controls
No positive or negative controls explicitly mentioned
Annotations
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