Animals were prepared for observation by immobilization in a 5 µL drop of 15 mM levamisole on a 4% agar pad. Worms were observed under 40×, 63×, and 100× objective lens using a Leica DM5500B compound microscope equipped with differential interference contrast optics and epifluorescence. Images were captured using a Hamamatsu C4742-95 digital camera controlled with Leica LAS © software.
To make a video of grinder turnover during L4 lethargus, we immobilized the animals against agarose pads using polystyrene nanoparticles (Kim, E., et al., 2013 (link)). Images were captured at 3.75 frames per second using an Imaging Source DMK 31BU03 camera on a Leica DM 2500 P upright microscope at 63× and analyzed using IC Capture 2.2 software (Imaging Source). To make the video, one out of every 36 images was used and played at 30 frames per second.
To make a video of grinder turnover during L4 lethargus, we immobilized the animals against agarose pads using polystyrene nanoparticles (Kim, E., et al., 2013 (link)). Images were captured at 3.75 frames per second using an Imaging Source DMK 31BU03 camera on a Leica DM 2500 P upright microscope at 63× and analyzed using IC Capture 2.2 software (Imaging Source). To make the video, one out of every 36 images was used and played at 30 frames per second.
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