TNF-α and IL-1β were detected in the rat eye tissue with EIU by immunohistochemical staining using rabbit polyclonal antibodies (TNF-α;bs-2081R, IL-1β;bs-6319R, Bioss, USA) and the streptavidin–biotin peroxidase technique. The procedure was performed under the same conditions for all sections as previously described.[13 (link)]
Immunohistochemical evaluations were performed using the extensity of the staining. The distribution (0.1: <25%, 0.4: 26%–50%, 0.6: 51%–75%, 0.9: 76%–100%) and intensity (0: no staining; +0.5: very little staining; +1: little staining; +2: medium staining; +3: very strong staining) of immune reactivity was used to obtain a histoscore (Histoscore = distribution × intensity).[14 (link)]
The SPSS statistical software package version 25.0 (SPSS Inc., Chicago, IL, USA) was used to analyze the data. The data were reported as mean ± standard deviations for each data set. A statistical significance was considered if P < 0.05. The statistical analyses of the data were performed using one-way analysis of variance test, Kruskal–Wallis test and post hoc analyses (Tukey test and pairwise comparisons using Bonferroni correction, respectively).
Immunohistochemical evaluations were performed using the extensity of the staining. The distribution (0.1: <25%, 0.4: 26%–50%, 0.6: 51%–75%, 0.9: 76%–100%) and intensity (0: no staining; +0.5: very little staining; +1: little staining; +2: medium staining; +3: very strong staining) of immune reactivity was used to obtain a histoscore (Histoscore = distribution × intensity).[14 (link)]
The SPSS statistical software package version 25.0 (SPSS Inc., Chicago, IL, USA) was used to analyze the data. The data were reported as mean ± standard deviations for each data set. A statistical significance was considered if P < 0.05. The statistical analyses of the data were performed using one-way analysis of variance test, Kruskal–Wallis test and post hoc analyses (Tukey test and pairwise comparisons using Bonferroni correction, respectively).
