In vivo Cytotoxicity Assay with CFSE-labeled Cells

As previously described [24 (link)], the mice were injected i.v. with a mixture of splenocytes labeled with CFSE (20 nM) and loaded with 100 nM SIINFEKL peptide. The spleen cells were labeled using a 10 mM CellTracker^TM Orange CMTMR (Life technologies), and they were not loaded with peptide. A total of 10 × 10⁶ cells per mouse were injected, with a mixture containing each target cell population. Splenocytes were collected 6 hours after injection of target cells. The percentage of killing was calculated using the formula described in [35 (link)].

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Zhang W., Yu X., Kwak M., Xu L., Zhang L., Yu Q, & Jin J.O. (2016). Maturation of dendritic cells by pullulan promotes anti-cancer effect. Oncotarget, 7(28), 44644-44659.

Publication 2016

CellTrackerTM Orange CMTMR

Cells Cfse Cmtmr Mouse Peptide Siinfekl peptide Spleen Target population

Corresponding Organization :

Other organizations : Shanghai Public Health Clinical Center, Stomatology Hospital, Peking University, Pukyong National University, Harvard University

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Variable analysis

independent variables

Injection of a mixture of splenocytes labeled with CFSE (20 nM) and loaded with 100 nM SIINFEKL peptide

dependent variables

Percentage of killing

control variables

Spleen cells labeled using a 10 mM CellTracker™ Orange CMTMR (Life technologies), and they were not loaded with peptide
A total of 10 × 10^6 cells per mouse were injected, with a mixture containing each target cell population
Splenocytes were collected 6 hours after injection of target cells

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