All cell lines (MDA-MB-157, MDA-MB-468, MDA-MB-231, HCC38, HCC1937, HS578T) were obtained from the Massachusetts General Hospital Center for Molecular Therapeutics bank and subjected to high-density SNP genotyping to confirm their identity. Cells were grown in RPMI medium (Lonza) supplemented with 10% FBS (Sigma) as well as 1% penicillin/streptomycin (Gibco), and were used up to a maximum of 20 passages following thawing. Cell lines were tested negative for Mycoplasma using MycoAlert Mycoplasma Detection Kit (Lonza). Cisplatin resistance state cells were generated using a previously reported protocol (13 (link)). Cisplatin (Enzo Life Sciences) was dissolved in 0.9% sodium chloride; PD0325901 (Selleck Chemicals) and gefitinib (Selleck Chemicals) were dissolved in DMSO.
Protocol detail
Establishing Cisplatin-Resistant Breast Cancer Cell Lines
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RPMI medium
FBS
penicillin/streptomycin
MycoAlert Mycoplasma Detection Kit
Cisplatin
PD0325901
gefitinib
Cell lines
Cells
Cisplatin
Dmso
Gefitinib
Mycoplasma
Pd0325901
Penicillin
Sodium chloride 0 9
Streptomycin
Therapeutics
Corresponding Organization : Harvard University
Other organizations : Broad Institute, Dana-Farber Cancer Institute, Kettering University, Memorial Sloan Kettering Cancer Center, University of North Carolina at Chapel Hill, University of California, San Francisco, Johns Hopkins University, Sidney Kimmel Cancer Center
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Variable analysis
independent variables
- Cisplatin resistance state
- Cell line identity confirmation
- Mycoplasma contamination status
- Cell culture medium (RPMI)
- Fetal bovine serum (FBS)
- Penicillin/streptomycin
- Maximum cell passage number (20)
- None specified
- None specified
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