Transposome-based DNA Tagmentation Protocol

For tagmentation, transposome was assembled as previously described^{39 (link)} using purified Tn5 protein and oligonucleotides purchased from IDT. Two hundred nanogram of genomic DNA was incubated with 2ul of assembled transposome at 55 degrees for 7 mins, and the product was cleaned up (20 μl) with a Zymo column (Zymo Research, #D4013). Tagmented DNA was used for the 1st PCR using PlatinumTM SuperFi DNA polymerase (Thermo) with i5 primer and gene-specific primers (Supplementary Table 3). Two different libraries were prepared for gDNA from each mouse with different combinations of primers (i5 + Locus_F [UDiTaS], i5 + Locus_R [UDiTaS]). The i7 index was added in the 2nd PCR and the PCR product was cleaned up with Ampure XP SPRI beads (Agencourt, 0.9X reaction volume). Completed libraries were quantified by Tapestation and Qubit (Agilent), pooled with equal amounts, and sequenced with 150 bp paired-end reads on an Illumina MiniSeq instrument.