Metabolic Profiling of Polar Compounds

Metabolic profiling of the polar phase was done as described (45 (link)) with minor modifications as described below. Briefly, analysis was performed using the Acquity I Class UPLC System combined with a mass spectrometer (Thermo Exactive Plus Orbitrap), which was operated in a negative ionization mode. The LC separation was done using the SeQuant Zic-pHilic (150 mm × 2.1 mm) with the SeQuant guard column (20 mm × 2.1 mm; Merck). There were two mobile phases performed: mobile phase A with acetonitrile and mobile phase B with 20 mmol/L ammonium carbonate plus 0.1% ammonia hydroxide in water. The flow rate was kept at 200 μL/minute and gradient as follows: 0 to 2 minutes 75% of B, 17 minutes 12.5% of B, 17.1 minutes 25% of B, 19 minutes 25% of B, 19.1 minutes 75% of B, and 19 minutes 75% of B.

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Goldman O., Adler L.N., Hajaj E., Croese T., Darzi N., Galai S., Tishler H., Ariav Y., Lavie D., Fellus-Alyagor L., Oren R., Kuznetsov Y., David E., Jaschek R., Stossel C., Singer O., Malitsky S., Barak R., Seger R., Erez N., Amit I., Tanay A., Saada A., Golan T., Rubinek T., Sang Lee J., Ben-Shachar S., Wolf I, & Erez A. (2023). Early Infiltration of Innate Immune Cells to the Liver Depletes HNF4α and Promotes Extrahepatic Carcinogenesis. Cancer Discovery, 13(7), 1616-1635.

Publication 2023

I Class UPLC System Exactive Plus Orbitrap SeQuant Zic-pHilic SeQuant guard column

Acetonitrile Ammonia Ammonium carbonate Ammonium hydroxide Carbonate Hydroxide

Corresponding Organization :

Other organizations : Weizmann Institute of Science, Tel Aviv University, Sheba Medical Center, Tel Aviv Sourasky Medical Center, Hadassah Medical Center, Sungkyunkwan University, Clalit Health Services

Top 5 similar protocols

Variable analysis

independent variables

Manipulation of the mobile phase composition

dependent variables

Metabolic profile of the polar phase

control variables

Acquity I Class UPLC System combined with a mass spectrometer (Thermo Exactive Plus Orbitrap) operating in negative ionization mode
LC separation using the SeQuant Zic-pHilic (150 mm × 2.1 mm) with the SeQuant guard column (20 mm × 2.1 mm; Merck)
Flow rate of 200 μL/minute
Gradient elution program as specified (0 to 2 minutes 75% of B, 17 minutes 12.5% of B, 17.1 minutes 25% of B, 19 minutes 25% of B, 19.1 minutes 75% of B, and 19 minutes 75% of B)

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