Synthesis and Purification of Antigenic Peptides

The antigenic peptides hgp100_(25–33) (KVPRNQDWL and KVPRNQDWLC-6-ahx-lysine (biotin)) [81 (link)], mgp100_25–33 (EGSRNQDWL), human gp100_280–288 (YLEPGPVTA and YLEPGPVTAC-6-ahx-lysine (biotin)), Trp1_222–229 (TAYRYHLL and TAYRYHLLC-6-ahx-lysine (biotin)), Trp2_180–188 (SVYDFFVWL and SVYDFFVWLC-6-ahx-lysine (biotin)) and MART1_(26–35) (ELAGIGILTV and ELAGIGILTVC-6-ahx-lysine (biotin)) were synthesized at the GlycO2peptide unit at our lab by solid phase peptide synthesis using Fmoc chemistry on a Symphony peptide synthesizer (Protein Technologies Inc., Tucson, AZ, USA). The linker 6-ahx and the special amino acid lysine(biotin) were purchased at Iris Biotech GmbH (Marktredwitz, Germany) The peptides were purified on a preparative Ultimate 3000 HPLC system (Thermo Fisher Scientific, Breda, the Netherlands) over a Vydac 218MS1022 C18 25 × 250mm column (Grace Davidson, Worms, Germany). Quality control was performed by UPLC-MS on a Ultimate 3000 UHPLC system (Thermo Fisher Scientific) hyphenated with a LCQ-Deca XP Iontrap ESI mass spectrometer (Thermo Finnigan, Waltham, MA, USA) using a RSLC 120 C18 Acclaim 2.2um particle 2.1 × 250 mm column and ionizing the sample in positive mode.

Free full text: Click here

van Dinther D., Lopez Venegas M., Veninga H., Olesek K., Hoogterp L., Revet M., Ambrosini M., Kalay H., Stöckl J., van Kooyk Y, & den Haan J.M. (2019). Activation of CD8+ T Cell Responses after Melanoma Antigen Targeting to CD169+ Antigen Presenting Cells in Mice and Humans. Cancers, 11(2), 183.

Publication 2019

Ultimate 3000 HPLC system Ultimate 3000 UHPLC system LCQ-Deca XP Iontrap ESI mass spectrometer

Amino acid Antigenic Biotin Hplc Human Iris Lysine Mart1 Peptides Protein Worms Ylepgpvta

Corresponding Organization : Vrije Universiteit Amsterdam

Other organizations : Medical University of Vienna

Top 5 similar protocols

Variable analysis

independent variables

Hgp100(25–33) (KVPRNQDWL and KVPRNQDWLC-6-ahx-lysine (biotin))
Mgp100(25–33) (EGSRNQDWL)
Human gp100(280–288) (YLEPGPVTA and YLEPGPVTAC-6-ahx-lysine (biotin))
Trp1(222–229) (TAYRYHLL and TAYRYHLLC-6-ahx-lysine (biotin))
Trp2(180–188) (SVYDFFVWL and SVYDFFVWLC-6-ahx-lysine (biotin))
MART1(26–35) (ELAGIGILTV and ELAGIGILTVC-6-ahx-lysine (biotin))

dependent variables

Not explicitly mentioned

control variables

The linker 6-ahx and the special amino acid lysine(biotin) were kept constant in the peptide synthesis process.

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!