Arabidopsis Protoplast Transformation in 96-well Plate

Protoplasts were generated from 3-day-old Arabidopsis Col-0 dark-grown cell suspension culture, and the transformations were carried out in small scale in 96 well plates with round-bottom (Roth) as previously described [30 (link)] using a Liquidator⁹⁶ Manual Pipetting system (Steinbrenner GmbH, Germany). The DNA was purified using either the Gene JET Plasmid Midiprep kit or the Maxiprep version (Thermo Scientific), eluting in water and adjusting the final concentration to 1μg/μl.

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Llorca C.M., Berendzen K.W., Malik W.A., Mahn S., Piepho H.P, & Zentgraf U. (2015). The Elucidation of the Interactome of 16 Arabidopsis bZIP Factors Reveals Three Independent Functional Networks. PLoS ONE, 10(10), e0139884.

Publication 2015

Gene JET Plasmid Midiprep kit Maxiprep

Arabidopsis Col 3 Culture cell Gene Plasmid Protoplasts

Corresponding Organization :

Other organizations : University of Tübingen, University of Hohenheim

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Variable analysis

independent variables

Cell suspension culture age (3-day-old)
Arabidopsis Col-0 ecotype
Transformation method (Liquidator^96 Manual Pipetting system)
DNA purification method (Gene JET Plasmid Midiprep kit or Maxiprep version)

dependent variables

Transformation efficiency (not explicitly mentioned)

control variables

Cell suspension culture growth conditions (dark-grown)
DNA concentration (1μg/μl)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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