Cultivation and Isolation of Cell Lines

SH-SY5Y cells (European Collection of Cell Cultures; Sigma-Aldrich) were cultivated in RPMI 1640 medium containing 2 g/l glucose and 5% or 10% FCS. HeLa cells (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH) were cultivated in Minimal Essential Medium with Earle's salts containing 10% FCS and 1% non essential amino acids. Primary cortical neurons were isolated from 1–4 d old mice as described previously [3] (link) and incubated with Neurobasal medium for 10–20 days before performing experiments. All cells were kept at 37°C with 5% CO₂ and 95% air.

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Parganlija D., Klinkenberg M., Domínguez-Bautista J., Hetzel M., Gispert S., Chimi M.A., Dröse S., Mai S., Brandt U., Auburger G, & Jendrach M. (2014). Loss of PINK1 Impairs Stress-Induced Autophagy and Cell Survival. PLoS ONE, 9(4), e95288.

Publication 2014

SH-SY5Y cells HeLa cells

Cell cultures Cells Cortical Essential amino acids European Glucose Hela cells Mice Neurons Salts

Corresponding Organization : University Hospital Frankfurt

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Variable analysis

independent variables

Cell culture conditions (5% or 10% FCS)
Cell types (SH-SY5Y, HeLa, primary cortical neurons)

dependent variables

Not explicitly mentioned

control variables

Cell culture media (RPMI 1640, Minimal Essential Medium, Neurobasal medium)
Incubation conditions (37°C, 5% CO2, 95% air)
Timeframe for primary cortical neuron experiments (10-20 days)

controls

Positive control: Not specified
Negative control: Not specified

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