Bile Acid Analysis in Bronchoalveolar Lavage Fluid

BALF samples were treated with equal volumes of Sputolysin^® (Calbiochem, Darmstadt, Germany) and processed as described previously [16 (link),26 (link)]. Each BALF sample was analysed blinded to patient data for the presence of 12 principal bile acids compared to purified reference standards. Cholic acid (CA), chenodeoxyCholic acid (CDCA), deoxyCholic acid (DCA), lithoCholic acid (LCA), ursodeoxyCholic acid (UDCA), glycodeoxyCholic acid (GDCA), taurochenodeoxyCholic acid, (TCDCA), taurodeoxyCholic acid (TDCA), tauroCholic acid (TCA), glycoCholic acid (GCA), taurolithoCholic acid (TLCA) were purchased from Sigma-Aldrich (Buchs, Switzerland) and tauroursodeoxyCholic acid (TUDCA) was purchased from Calbiochem. All chemicals used were HPLC grade.

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Caparrós-Martín J.A., Flynn S., Reen F.J., Woods D.F., Agudelo-Romero P., Ranganathan S.C., Stick S.M, & O’Gara F. (2020). The Detection of Bile Acids in the Lungs of Paediatric Cystic Fibrosis Patients Is Associated with Altered Inflammatory Patterns. Diagnostics, 10(5), 282.

Publication 2020

Cholic acid chenodeoxycholic acid deoxycholic acid lithocholic acid ursodeoxycholic acid glycodeoxycholic acid taurochenodeoxycholic acid taurodeoxycholic acid taurocholic acid glycocholic acid taurolithocholic acid

Bile acids Chenodeoxycholic acid Cholic acid Deoxycholic acid Glycocholic acid Glycodeoxycholic acid Hplc Lithocholic acid Patient Sputolysin Taurochenodeoxycholic acid Taurocholic acid Taurodeoxycholic acid Taurolithocholic acid Tauroursodeoxycholic acid Ursodeoxycholic acid

Corresponding Organization : The Kids Research Institute Australia

Other organizations : Royal Children's Hospital, Murdoch Children's Research Institute, University of Melbourne, Princess Margaret Hospital for Children

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Variable analysis

independent variables

Treatment of BALF samples with equal volumes of Sputolysin® (Calbiochem, Darmstadt, Germany)

dependent variables

Presence of 12 principal bile acids in each BALF sample compared to purified reference standards

control variables

Each BALF sample was analysed blinded to patient data

controls

Purified reference standards of the following bile acids were used: cholic acid (CA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), lithocholic acid (LCA), ursodeoxycholic acid (UDCA), glycodeoxycholic acid (GDCA), taurochenodeoxycholic acid, (TCDCA), taurodeoxycholic acid (TDCA), taurocholic acid (TCA), glycocholic acid (GCA), taurolithocholic acid (TLCA), and tauroursodeoxycholic acid (TUDCA)

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