Standardized Detection of Bacillus cereus

The qualitative and quantitative detection of B. cereus were performed according to National Food Safety Standard (The Hygiene Ministry of China, 2010a ) with minor modification. In brief, 25 ml of sample was mixed and homogenized with 225 ml Trypticase-soy-polymyxin (TSB) broth (Huankai, China) at 30°C for 48 h. Then cultures were streaked on the Mannitol-egg yolk-polymyxin (MYP) agar plate (Selective media; Huankai, China) and Chromogenic plate (Huankai, China) and incubated at 30°C for 24 h. Colonies with pink sparkle in blue or blue-green precipitation on Chromogenic plate were picked for further biochemical identification using the B. cereus biochemistry assessor (Huankai, China). The quantitative detection assay was conducted by B. cereus most probable number (MPN) counting method in Food Safety Standards (The Hygiene Ministry of China, 2010a ).

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Gao T., Ding Y., Wu Q., Wang J., Zhang J., Yu S., Yu P., Liu C., Kong L., Feng Z., Chen M., Wu S., Zeng H, & Wu H. (2018). Prevalence, Virulence Genes, Antimicrobial Susceptibility, and Genetic Diversity of Bacillus cereus Isolated From Pasteurized Milk in China. Frontiers in Microbiology, 9, 533.

Publication 2018

Agar Assay Chromogenic Egg yolk Mannitol Polymyxin Trypticase soy

Corresponding Organization :

Other organizations : South China Sea Institute Of Oceanology, University of Chinese Academy of Sciences, Institute of Oceanology, Guangdong Academy of Sciences, Institute of Microbiology, Jinan University, University of Jinan, South China Agricultural University

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Variable analysis

independent variables

Incubation temperature (30°C)
Incubation duration (48 h for pre-enrichment, 24 h for agar plates)

dependent variables

Qualitative detection of B. cereus (based on colony characteristics on selective media)
Quantitative detection of B. cereus (most probable number counting method)

control variables

Sample volume (25 ml)
Enrichment broth (Trypticase-soy-polymyxin (TSB) broth)
Selective media (Mannitol-egg yolk-polymyxin (MYP) agar, Chromogenic plate)

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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