Antibodies for Hepatitis E Virus Detection

Rabbit monoclonal antibody (MAb) C29F4 against the HA epitope was purchased from Cell Signaling Technology (Leiden, The Netherlands). Mouse MAbs against HEV ORF2 (1E6), MAVS (Adri-1), GM130 (clone 35), and β-actin (AC-15) were from Millipore (Burlington, MA), AdipoGen (San Diego, CA), BD Biosciences (San Jose, CA), and Sigma-Aldrich (St. Louis, MO), respectively. Mouse MAbs against ERGIC-53 (OTI108), PDI (1D3), and CLIMP63 (G1/296) were from Enzo Life Sciences (Farmingdale, NY), and MAbs against CD63 (MX491295) and CD151 (11G5a) were from Santa Cruz Biotechnology (Dallas, TX). Recombinant mouse antibody MRB198 against genotype 3 HEV ORF3 protein have been described previously (11 (link)). Alexa Fluor 488 and Alexa Fluor 694 anti-mouse IgG as well as Alexa Fluor 594 anti-rabbit IgG secondary antibodies were from Thermo Fisher Scientific. Horseradish peroxidase-conjugated anti-mouse IgG and anti-rabbit IgG secondary antibodies were from GE Healthcare (Chicago, IL) and Agilent Technologies (Santa Clara, CA), respectively.

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Szkolnicka D., Pollán A., Da Silva N., Oechslin N., Gouttenoire J, & Moradpour D. (2019). Recombinant Hepatitis E Viruses Harboring Tags in the ORF1 Protein. Journal of Virology, 93(19), e00459-19.

Publication 2019

β-actin (AC-15) Alexa Fluor 594 anti-rabbit IgG Horseradish peroxidase-conjugated anti-mouse IgG and anti-rabbit IgG secondary antibodies

Actin Alexa 594 Alexa fluor 488 Anti igg Antibodies Antibody Cd151 Clone Epitope Genotype Horseradish peroxidase Mabs Mavs Mouse Rabbit Recombinant protein

Corresponding Organization :

Other organizations : University of Lausanne, University Hospital of Lausanne

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Variable analysis

independent variables

Rabbit monoclonal antibody (MAb) C29F4 against the HA epitope
Mouse MAbs against HEV ORF2 (1E6), MAVS (Adri-1), GM130 (clone 35), and β-actin (AC-15)
Mouse MAbs against ERGIC-53 (OTI108), PDI (1D3), and CLIMP63 (G1/296)
Mouse MAbs against CD63 (MX491295) and CD151 (11G5a)
Recombinant mouse antibody MRB198 against genotype 3 HEV ORF3 protein

dependent variables

Not explicitly mentioned

control variables

Alexa Fluor 488 and Alexa Fluor 694 anti-mouse IgG as well as Alexa Fluor 594 anti-rabbit IgG secondary antibodies
Horseradish peroxidase-conjugated anti-mouse IgG and anti-rabbit IgG secondary antibodies

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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