Characterization of F. oxysporum mutants

Wild type F. oxysporum f.sp. lycopersici (Fol) strain 4287 and mutant strains used in this study are listed in Supplementary Table S1. Vegetative growth rate was determined using potato dextrose agar (PDA) and complete medium (CM) at 28°C for 5 days (Li et al., 2019b (link)). Conidiation was assayed as previously reported (Li et al., 2019b (link)). The conidial morphology of wild type and mutants was examined using a Zeiss LSM 710 confocal microscope with a 63/1.2 NA C-Apochromat oil immersion objective (Zeiss, Oberkochen, Germany). Liquid CM was used to grow cultures for extraction of genomic DNA and total RNA.

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Li B., Mao H.Y., Zhang Z.Y., Chen X.J, & Ouyang S.Q. (2019). FolVps9, a Guanine Nucleotide Exchange Factor for FolVps21, Is Essential for Fungal Development and Pathogenicity in Fusarium oxysporum f. sp. lycopersici. Frontiers in Microbiology, 10, 2658.

Publication 2019

LSM 710 confocal microscope 63/1.2 NA C-Apochromat oil immersion objective

A 2 c A a 1 Agar Confocal microscope Conidial Dextrose Genomic Immersion Potato Strains

Corresponding Organization : Yangzhou University

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Variable analysis

independent variables

Strain type (wild type vs. mutant)

dependent variables

Vegetative growth rate
Conidiation (spore production)
Conidial morphology

control variables

Culture medium (PDA, CM)
Temperature (28°C)
Culture duration (5 days)

controls

No positive or negative controls were explicitly mentioned in the input.

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