Quantifying Collagen Autoantibodies in Mice

Circulating levels of mouse anti-collagen antibodies (auto-antibodies) and bovine anti-collagen antibodies was assessed by ELISA in serum collected from mice in the CIA model. A mouse anti-mouse type II collagen IgG antibody assay kit and mouse anti-bovine type II collagen IgG antibody assay kit was used, according to the manufacture's protocol (Chondrex Inc. Redmond, WA, USA). The antibody concentrations in the samples were calculated by comparison with the optical density (OD) values of standard anti-CII antibody (units/ml) provided in each of the assay kits as previously described (24 (link), 26 (link)).

Free full text: Click here

Hemshekhar M., Piyadasa H., Mostafa D., Chow L.N., Halayko A.J, & Mookherjee N. (2020). Cathelicidin and Calprotectin Are Disparately Altered in Murine Models of Inflammatory Arthritis and Airway Inflammation. Frontiers in Immunology, 11, 1932.

Publication 2020

mouse anti-mouse type II collagen IgG antibody assay kit mouse anti-bovine type II collagen IgG antibody assay kit

Anti antibodies Anti antibody Anti igg Antibodies Antibody Assay Bovine Chondrex Collagen Elisa Igg antibody Mouse Serum Type ii collagen Values

Corresponding Organization : Children's Hospital Research Institute of Manitoba

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Circulating levels of mouse anti-collagen antibodies (auto-antibodies)
Circulating levels of bovine anti-collagen antibodies

control variables

None explicitly mentioned

controls

Positive control: Standard anti-CII antibody (units/ml) provided in each of the assay kits
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!