Glucose, Insulin, and Lipid Metabolic Assays

Glucose and insulin tolerance tests were performed by blood sampling after an intraperitoneal injection of glucose (1 mg/g; Sigma-Aldrich, UK) or human recombinant insulin (0.75 unit/kg: Actrapid; Novo Nordisk, Bagsvaerd, Denmark), as previously described (5 (link), 6 (link), 30 (link), 32 (link), 33 (link)). Glucose concentrations were determined in whole blood using a portable meter (Roche Diagnostics, Burgess Hill, UK). Plasma insulin concentrations were determined by enzyme-linked immunoassay (Ultrasensitive mouse ELISA; CrystalChem, Downers Grove, IL). Triglycerides and total cholesterol were quantified as described previously (8 (link)). Liver function was assessed by measuring blood serum levels of alanine aminotransferase (ALT) and aspartate transaminase (AST) (74707, 74032, Advia Chemistry, Siemens).

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Maqbool A., Watt N.T., Haywood N., Viswambharan H., Skromna A., Makava N., Visnagri A., Shawer H.M., Bridge K., Muminov S.K., Griffin K., Beech D.J., Wheatcroft S.B., Porter K.E., Simmons K.J., Sukumar P., Shah A.M., Cubbon R.M., Kearney M.T, & Yuldasheva N.Y. (2020). Divergent effects of genetic and pharmacological inhibition of Nox2 NADPH oxidase on insulin resistance-related vascular damage. American Journal of Physiology - Cell Physiology, 319(1), C64-C74.

Publication 2020

Actrapid Ultrasensitive mouse ELISA

Alanine aminotransferase Aspartate transaminase Blood Blood serum Cholesterol Diagnostics Elisa Enzyme immunoassay Glucose Human Insulin Intraperitoneal injection Liver Mouse Plasma Tolerance Triglycerides

Corresponding Organization : University of Leeds

Other organizations : Tashkent Pediatric Medical Institute, British Heart Foundation, King's College London

Top 5 similar protocols

Variable analysis

independent variables

Intraperitoneal injection of glucose (1 mg/g; Sigma-Aldrich, UK)
Intraperitoneal injection of human recombinant insulin (0.75 unit/kg: Actrapid; Novo Nordisk, Bagsvaerd, Denmark)

dependent variables

Glucose concentrations in whole blood
Plasma insulin concentrations
Triglycerides
Total cholesterol
Alanine aminotransferase (ALT)
Aspartate transaminase (AST)

control variables

Blood sampling procedure
Portable meter for glucose determination
Enzyme-linked immunoassay for insulin determination
Previously described methods for triglyceride and cholesterol quantification (8)
Previously described methods for liver function assessment (74707, 74032, Advia Chemistry, Siemens)

positive controls

None specified

negative controls

None specified

Annotations

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