DMEM culture medium, fetal
bovine serum (FBS), 0.25% Trypsin, phosphate-buffered saline (PBS),
and penicillin–streptomycin were from Gibco (Grand Island,
NY). Murine macrophage line RAW264.7 cells was obtained from the cell
bank of the Chinese Academy of Sciences (Shanghai). Human ox-LDL (oxidized
low-density lipoproteins), HDL (high-density lipoprotein), and Dil-ox-LDL
(oxidized low-density lipoprotein, labeled with 1,1′-dioctadecyl–3,3,3′,3′-tetramethylindocarbocyanine
perchlorate) were purchased from Yiyuan biotechnologies (Guangzhou,
China). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium
bromide), 25-NBD cholesterol (25-[N-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-methyl]amino]-27-norcholesterol),
and oil red O were purchased from Sigma-Aldrich (St Louis, MO). A
4% paraformaldehyde fix solution was from Jingxin Biotechnologies
(Guangzhou, China). GAPDH was purchased from Cell Signaling Technology
(Beverly, MA). ABCA1 (ATP-binding cassette transporter A1), ABCG1
(ATP-binding cassette transporter G1), SRA1 (scavenger receptor class
A type I), SRB1 (scavenger receptor class B type I), and CD36 were
all purchased from NOVUS Biologicals (Littleton, CO).
bovine serum (FBS), 0.25% Trypsin, phosphate-buffered saline (PBS),
and penicillin–streptomycin were from Gibco (Grand Island,
NY). Murine macrophage line RAW264.7 cells was obtained from the cell
bank of the Chinese Academy of Sciences (Shanghai). Human ox-LDL (oxidized
low-density lipoproteins), HDL (high-density lipoprotein), and Dil-ox-LDL
(oxidized low-density lipoprotein, labeled with 1,1′-dioctadecyl–3,3,3′,3′-tetramethylindocarbocyanine
perchlorate) were purchased from Yiyuan biotechnologies (Guangzhou,
China). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium
bromide), 25-NBD cholesterol (25-[N-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-methyl]amino]-27-norcholesterol),
and oil red O were purchased from Sigma-Aldrich (St Louis, MO). A
4% paraformaldehyde fix solution was from Jingxin Biotechnologies
(Guangzhou, China). GAPDH was purchased from Cell Signaling Technology
(Beverly, MA). ABCA1 (ATP-binding cassette transporter A1), ABCG1
(ATP-binding cassette transporter G1), SRA1 (scavenger receptor class
A type I), SRB1 (scavenger receptor class B type I), and CD36 were
all purchased from NOVUS Biologicals (Littleton, CO).
