The MSC-Exos was labeled with a lipophilic fluorescent dye (DiR; Invitrogen) to detect their biodistribution in vivo using an in vivo fluorescence imager (IVIS Spectrum). To evaluate the effect of exosomes on BMSCs in vivo, BMSCs labeled with enhanced green fluorescent protein using lentivirus (Cyagen Biosciences) were injected into the humeral of mice at a density of 1×106. After 2 weeks of treatment, the mice were sacrificed for immunofluorescence staining as previously described.27 (link)
Primary antibodies anti-GFP (1:400) and secondary antibodies (1:500) were purchased from Abcam. Finally, the slices were sealed with DAPI (4′,6-diamidino-2-phenylindole) and observed with a fluorescence microscope (Zeiss).
Primary antibodies anti-GFP (1:400) and secondary antibodies (1:500) were purchased from Abcam. Finally, the slices were sealed with DAPI (4′,6-diamidino-2-phenylindole) and observed with a fluorescence microscope (Zeiss).
